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酵母双杂交用缺陷型培养基SD/Leu/Trp/His/Ade(不含agar)1L

  • 品牌:FCNCS

A. Minimal Liquid Media (Broth)prefix = "o" ns = "urn:schemas-microsoft-com:office:office"

1. Dissolve a package media powder of a Minimal SD Base Medium and the appropriate dropout supplement to 0.5 L deionized H2O.

2. Adjust media pH to 5.8.

3. Autoclave at 121oC for 15min.

4. Store medium at room temperature.

 

B. Minimal Plating Media with Agar

1. Dissolve a package media powder of a Minimal SD Base Medium and the appropriate dropout supplement to 0.5 L deionized H2O.

2. Adjust media pH to 5.8.

3. Then add 2% agar, the agar will not dissolve until it is autoclaved.

4. Autoclave at 121oC for 15min. Cool to the temperature which your hand can endure it and then pouring plates.

5. Storage plated medium at room temperature. Store plates inverted at 4 oC.

6. Important: Verify that the agar you used contain no other nutriment!

Yeast Media

Cat. No.

Package Size

Storage Condition

YPDA Broth

M182

1 L

RT

YPDA with Agar

M183

1 L

RT

SD Base

M184

10 L

RT

SD/-Leu

M185

0.5 L

RT

SD/-Trp

M186

0.5 L

RT

SD/-Ura

M187

0.5 L

RT

SD/-Leu/-Trp

M188

0.5 L

RT

SD/-Leu/-Trp/-His

M189

0.5 L

RT

SD/-Leu/-Trp/-His/-Ade

M190

0.5 L

RT

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